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| underwater weighing, swinenutrition, dvds, ink jets, laser cartridge, fitkid, murata, electronics, body weight, caliper, assisted, automotive, absolution, xerox, body fat monitor, lasersmith, help, cancer, skin, tennis elbow, toshiba, | To decipher the modes of function of the newly identified genes, we are establishing genetic interaction networks between tanita bf 682 various mutants and RNAi clones that cause fat reduction or fat increase. tanita bf 682 We take advantage of suppressor/enhancer screens to disentangle the complex feedback loops that affect body fat. Fat regulatory genes can be broadly classified as those that impact food intake or energy expenditure. Thus, we measure each of these parameters in the mutant animals or animals exposed to each fat regulatory RNAi clone. Fusion of GFP tags to fat regulatory proteins allows for monitoring cellular expression and tanita bf 682 subcellular localization of each of the fat regulatory genes. These GFP fusions classify the genes directly involved in fat storage and utilization or those that function as neuronal regulators of feeding and energy expenditure. |
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| These body weight fat regulatory genes include receptors, channels, signal transduction molecules, transcription and translation factors, vesicular transporters, metabolic enzymes, and a number of genes with unassigned functions. The shared ancestry of the known mammalian and worm fat regulatory genes suggest that many of these newly identified genes may also function in human fat regulation. Our goals are i) to understand the molecular functions and modes of regulation of the newly identified genes, ii) to understand the principles that govern how body weight hundreds of genes whose products are expressed in multiple tissues network to regulate a complex physiological body weight process, iii) to delineate the neuronal networks that regulate food intake and energy expenditure in worms, and iv) to extend these findings to identify mammalian fat and obesity genes and analyze how misregulation of these genes result in obesity associated diseases such as diabetes. |
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