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Subjects and Methods Top Abstract Introduction Subjects and Methods Results Discussion References Subjects The study population consisted of 46 lean and obese human subjects of mixed ethnicity recruited by newspaper advertisement, 18 individuals with a bed & bath diagnosis of Prader-Willi syndrome confirmed by chromosomal analysis, and 189 Japanese-American men and women. Clinical characteristics and mean plasma leptin levels of the three study groups are summarized in Table 1. Obesity was defined as a BMI > 27.3 for males and 27.8 for females (9). Subjects had normal physical examinations, and with the exception of Prader-Willi individuals, bed & bath whose food consumption was monitored to varying degrees in a supervised care environment, subjects were not actively limiting caloric intake, using medications, or exercising in an effort to lose weight at the time of study.
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