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| hewlett packard, school dinners, breeding, cardiosport, web, sprafka, online, open access, dataproducts, pc calculatorscontains 9 articles regarding body fat calculator., and providing..., aspartate aminotransferase, thurnham, oce, minerals, chicago pile 1, buffaloes, | Fat regulatory genes can be broadly classified as those that impact food intake or energy expenditure. Thus, we measure each of these parameters in travel awards the mutant animals or animals exposed to each fat regulatory RNAi clone. Fusion of GFP tags to fat regulatory proteins allows travel awards for monitoring cellular expression and subcellular localization of each of the fat regulatory genes. These GFP fusions classify the genes directly involved in fat storage and utilization or those that function as neuronal regulators of feeding and energy expenditure. These experiments categorize the RNAi clones into subsets travel awards with related functions. For example, kinases within a group would be likely to phosphorylate the metabolic enzymes or transcription factors of the same group. Moreover, we can determine whether the expression or localization of a given fat regulatory gene is regulated by extrinsic or intrinsic signals such as fat levels, food, developmental stage, and other fat regulatory genes. |
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| The shared ancestry of the known mammalian and worm fat regulatory genes suggest that many of these newly identified genes thurnham may also function in human fat regulation. Our goals are i) to understand the molecular functions and modes of regulation of the newly identified genes, thurnham ii) to understand the principles that govern how hundreds of genes whose products are expressed in multiple tissues network to regulate a complex physiological process, iii) to delineate the neuronal networks that regulate food intake and thurnham energy expenditure in worms, and iv) to extend these findings to identify mammalian fat and obesity genes and analyze how misregulation of these genes result in obesity associated diseases such as diabetes. To decipher the modes of function of the newly identified genes, we are establishing genetic interaction networks between various mutants and RNAi clones that cause fat reduction or fat increase. We take advantage of suppressor/enhancer screens to disentangle the complex feedback loops that affect body fat. |
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